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KMID : 0358619950290020243
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Korean Journal of Physiology
1995 Volume.29 No. 2 p.243 ~ p.250
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Interaction between Cholecystokinin and Secretin in Isolated Rat Pancreatic Acini
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Yoon Shin-Hee
Hahn Sang-June
Sim Sang-Soo
Rhie Duck-Joo
Song In-Young
Baek Hye-Jung
Kim Myung-Suk
Jo Yang-Hyeok
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Abstract
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A possible potentiation between cholecystokinin (CCK) and secretin in amylase secretion from isolated rat pancreatic acini was investigated. Combined treatment of acini with secretin and CCK at low concentrations, which are known to be physiological, resulted in enzyme secretion larger than the arithmetic sum of their separate effects. Such a potentiating effect also occurred between secretin and A23187 (Ca ionophore), between forskolin (adenylate cyclase activator) and CCK, and between forskolin and A23187. Staurosporin (protein kinase C inhibitor) and W7 (calmodulin antagonist) inhibited markedly the potentiated amylase release induced by the agonists, but KT5720 (protein kinase A inhibitor) did not affect the potentiated amylase release. Therefore, we concluded that the action of CCK in a physiological concentration is potentiated by secretin in a physiological concentration range and vice versa, and that the intracellular mechanism necessary for the potentiation is associated with . However, it is uncertain what mechanisms are involved in potentiation of amylase release after CAMP and .
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KEYWORD
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Calcium, cAMP, Cholecystokinin, Pancreatic amylase, Potentiation, Secretin
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